R&D activity:

Scientific programmes

1. Project title: Validation and standardization of diagnostic Polymerase Chain Reaction for detection of foodborne pathogens (acronym FOOD-PCR). EU RTD Frame Program V Quality of Life and Management of Living Resources (2000-2003). Official representative: Mihhail Sudakov; Jüri Kumar, Silva Sütt.

2. Project title: Molecular epidemiology of non-tuberculous mycobacterioses and immunomodulating and protective effect of secretory antigens of Mycobacterium avium. No B 1457 ABMG 00. 2000-2004. Ministry of Education. Principal investigator Mihhail Sudakov; senior personnel: researchers Silva Sütt, Sirje Kokassaar; lab personnel: Malle Toom.

3. Project title: Programme of basic financing and elaboration the theoretical and technological principles for diagnostics, prophylactics of farm animals and birds diseases, and the technologies of remedies (1994-1996). Principal investigator: Jüri Kumar.

 

Grants of the Estonian Science Foundation

Grant No 6002: Ecological aspects of etiology and spread of Mycobacterioses and investigation of NTM transfer factors. Estonian Science Foundation. 2004-2007. Grant-holder: Mihhail Sudakov, DVM, PhD, researchers: Silva Sütt, Sirje Kokassaar , Liidia Häkkinen.

Grant No 5403: Peptides, adaptogens and potentiation of vaccines. 2001-2004. Grant-holder Jüri Kumar PhD, researchers Ülo Pavel DSc (Biol), Tiiu Schattschneider PhD, Mari-Ann Kumar PhD.

Grant No 4471: Molecular epidemiology and immunogenesis of animal tuberculosis, paratuberculosis and NTM-mycobacterioses. 2000-2003. Grant-holder: Mihhail Sudakov, DVM, PhD, researchers: Silva Sütt, Sirje Kokassaar, Liidia Häkkinen.

Grant No 3216: The induction of antibodies agonist various serotypes of Escherichia coli in cows milk for protection of man from coliinfection (1998-2001). Grant-holder Juhan Simovart DSc (Vet); Researcher - doctorant Jnga Vainumäe.

Grant No 2788: Potentiation of vaccines (1997-2000). Grant-holder Jüri Kumar PhD, researchers Ülo Pavel DSc (Biol), Tiiu Schattschneider PhD, Mari-Ann Kumar PhD.

Grant No 2172: The research of influence of biological, immunological and immunomodulative properties of atypical mycobacteria on the mycobacterial process in animals (1996-1999). Grant-holder: Mihhail Sudakov, DVM, Ph.D., Senior personnel: Nikolai Kozlov prof. DSc (Vet), Liidia Häkkinen, researcher; Mari-Ann Kumar, researcher, Ph.D

Grant No 1956: Investigation of Salmonellae spread peculiarities in domestic fowls and humans in Estonia, biological characteristics and drug resistance of the most spread Salmonella serotypes (1996-1999). Grant-holder Helle-Evi Simovart PhD (Vet).

Grant No 1910: Isolation, cultivation, characterization and estimation of virulence of canine distemper virus (1996-1997). Grant-holder Juhan Simovart DSc (Vet); researcher Kalmer Karpson MS.

 

Applied research activities

Project title:
The application of molecular methods for detection of foodborne pathogens and non-tuberculous mycobacteria. 2002. Principal investigator: Jüri Kumar DVM, PhD.

Project title:
The application of molecular methods for detection of Campylobacter sp. in food. 2001. Principal investigator: Jüri Kumar DVM, PhD.

Project title:
Composing, editing and publishing of booklet “Zoonooses and food hygiene”. 2001. Principal investigator: Jüri Kumar DVM, PhD.

Project title:
Improvement and standardization of PCR-diagnostics of foodborne infections, transmitted with nutrition. 2000. Principal investigator: Jüri Kumar DVM, PhD.

Project title:
Starting the production of the ringworm vaccine of animals. 2000. Principal investigator: Jüri Kumar DVM, PhD.

Project title::
Improvement of differential diagnostics and control in animal tuberculosis and paratuberculosis. 1999. Principal investigator: Mihhail Sudakov, DVM, PhD.

Project title:
Improvement of methods of prophylaxis and diagnostics some zoonoses. 1998. (Estonian Innovation Foundation borrow short). Principal investigator: Jüri Kumar DVM, PhD.

 

Culture collections

In Estonian Agribiocentre there are collected strains of pathogenic and opportunist microbe isolates (Mycobacterium, Pasteurella, Salmonella, Escherichia coli, Erysipelothrix, Streptococcus, Staphylococcus, Trichophyton) and also isolates of lactobacilli (228 strains). Besides the local strains there are some reference strains from Moscow Control Institute of Biological and Medicinal Preparations, from the Russian Control Institute of Veterinary Preparations.
The cultures of microbes are stored in lyophilised state and on medium Protect Bacterial Preservers at To -20C. Bank of the strains will be utilized in scientific research, diagnostics and in production of biological preparations.

 

Publications

  1. Kumar J. (1997). On applied microbiological studies.- Põllumajandus: 12, pp. 8-9 (in Estonian).

  2. Karus A., Pavel Ü., Kumar J. (1998). The isolation of neuropeptide fractions from pig brain cortex and hypothalamus plus hypophysis.- Book of Abstracts of the 49th Ann. Meet. EAAP. Warsaw. Poland, 24-27 August 1998, p. 152.

  3. Kumar J., Karus A., Schattchneider T., Kumar A.-M., Kaljo A., Pavel Ü. (1999). On immunoprotective effect of cerebral neuropeptides.- Book of Abstracts of the 50th Ann. Meet. EAAP. Zürich, Switzerland, 22-26 August, 1999, p. 168. Full paper 7 p. available electronically at www.WageningenPers.nl

  4. Kumar J., Karus A., Schattschneider T., Kumar M.-A., Pavel Ü. (2000). Porcine Brain Cortex Peptides and Mouse Resistance.- Veterinaarmeditsiin' 2000, pp. 49-51.

  5. Kumar J., Karus A., Schattschneider T., Kumar M-A., Pavel Ü. (2000). Porcine Brain Cortex Peptides and Mouse Resistance.- Book of Abstracts of the 51st Ann. Meet. EAAP. The Hague, The Netherlands, 21-24 August, 2000, p.232.

  6. Kumar J, Lindjärv R., Rae K. (1998). Immunogenic studies of swine inactivated erysipelas vaccine “Erysvac II”.- Veterinaarmeditsiin’98, pp. 122-128 (in Estonian).

  7. Kumar J., Pavel Ü. (2000). Vaccines and Animal Infectious Diseases.- Maamajandus (accepted, in press; in Estonian).

  8. Kumar J., Pavel Ü., Lindjärv R., Schattschneider T. (2000). Autogenous bacterins in veterinary practice.- Current Issues in Veterinary Medicine. Jelgava, Latvia, September 29 , Procedineding, pp. 95-99.

  9. Kumar, J., Sudakov, M. (2000). Animal tuberculosis and Mycobacterioses stadies in Estonian Agribiocentre.- Põllumajandus, pp. 5-6 (in Estonian).

  10. Kumar J., Karus A., Pavel Ü., Schattschneider T., Kumar M.-A. Porcine Cerebral Neuropeptides and Resistance.- Reproductive Failure in Farm Animals (T.Tiirats ed). Proc.Symp. Estonian Agric.Univ. June 14-15, Tartu CRU Report 14, Uppsala, 2001, 54-59.

  11. Lindjärv R., Kalda O., Kumar J., Rae K. (1998). Immunogenic studies of swine inactivated erysipelas vaccine “Erysvac II”.- Veterinaarmeditsiin’98, pp. 128-133 (in Estonian).

  12. Lochmiller R. L., Pavel Ü. (1998). Important Immunotoxicological Considerations in Veterinary Ecology and Food Hygiene.- Acta Veter. Baltica, pp. 2-8.

  13. Pavel Ü., Kumar J., Karus A., Schattschneider T. Porcine brain cortex neuropeptides enhance the early phase of antibody synthesis in the vaccinated mouse.- Journ. of Agricultural Science, 2001, 12 (3): 189-192

  14. Pavel Ü., Reintam E., Karus A. 3. Haigusresistentsuse pärilikkuse alused.- Eesti Loomaarstlik Ringvaade, 2001, Nr. 1, lk. 5-7.

  15. Pavel Ü. (1999). On the Priorities in Veterinary Biology and Veterinary Medicine.- Veterinaarmeditsiin'99, pp. 111-113.

  16. Pavel Ü. On veterinary biology (1998).- Veterinaarmeditsiin'98, pp. 90-94.

  17. Pavel Ü. (1998). Veterinary biology.- Transactions of the Estonian Acad. Agricult. Society, No 7, pp. 81-84.

  18. Pavel Ü. (1999). Veterinary Biology.- Veterinaameditsiin' 99 pp. 109-110.

  19. Pavel Ü., Karus A., Kumar J. (2000a). Veterinary Biology: Adaptation and Brain.- Veterinaarmeditsiin '2000, pp. 64-66 (in Estonian).

  20. Pavel Ü., Karus A., Kumar J. (1999). Veterinary biology: resistance and fertility.- Dairy Production in Estonia - Today and Tomorrow. Proc. Symp. at Estonian Agric. Univ., Tartu /Uppsala. CRB Report 7, pp. 104-105.

  21. Pavel Ü., Kolk A. (1998). Problems in Veterinary Ecology.-Trans. Estonian Agric. Acad. Soc., pp. 85-86 (in Estonian).

  22. Pavel Ü., Kumar J., Karus A. (2000). A possible research model for specific prophylactics and treatment of bacterial pneumonia and diarrhoea in young animals.- 2nd Annu Sci. meeting of the European Society of Veterinary Clinical Pathology: Toulouse, France, July 21, 2 p.

  23. Pavel Ü., Kumar J.,. Lochmiller R. L (2000 b). Insights into Veterinary Biology: Genetics to Ecology. - Acta Veter. Baltica, pp. 2-10.

  24. Pavel Ü., Schattschneider T., Kumar J., Kumar M.-A., Karus A. (2000). Effect of porcine brain cortex neuropeptides on blood leukocyte profile in vaccinated mice.- Feeding, Metabolism, and Infections in Farm Animals with Special Reference on Reproduction. Symp. Tartu, Uppsala, CRU Report 11, February 25, pp.70-74.

  25. Pavel Ü., Kumar J., Karus A. Schattschneider T. Porcine brain cortex neuropeptides enhance the early phase of antibody synthesis in the vaccinated mouse.- Journ. Agricultural Science, 2001, 12, 3, 189-192.

  26. Pavel Ü., Kumar J. Veterinary biology: 4. Vaccines and animal’s immune response.- The Estonian Veterinary Review, 2001,3/4, 97-101.(in Estonian)

  27. Pavel Ü., Kumar J., Karus A. A possible research model for specific prophylaxis and treatment of bacterial pneumonia and diarrhea in young animals.- ESVCP Bulletin, 2001, 1, 1, 20.

  28. Simovart H.-E., Simovart J. (1998). Salmonella enteritidis ifection: Emidemiological situation and routes of transmission in Estonian layer floks.- A Spicial Symposium Arranged on Behaff of the Baltic Society for Parasitology and the Scandinavian Society for Parasitology. Vilnius, p. 70.

  29. Судаков М., Симоварт Ю., Козлов Н. (1996). Эпизоотическая ситуация по микобактериозам животных в Эстонии.- Тез. Междунар. докл. конф. посвященной 100-летию Курской биофабрики: Курск 27-30 августа 1996. Курск, c. 312-313.

  30. Судаков, М., Хяккинен, Л., Кумар, Ю., Никопенсиус, Т., Торосян, А., Крюнер, А. (1999). Сравнительный анализ результатов ПЦР, ДНК-зонда и конвенциональных методов идентификации Mycobacterium bovis и Mycobacterium avium complex. Проблемы инфекционных и инвазионных болезней в животноводстве на современном этапе: Тез. докл. Междунар. конф., посв. 80-летию Московской ветеринарной академии.- МВА, c. 220.

  31. Sudakov M., Häkkinen L., Sütt S. Mycobacterium avium - mükobakterioos metsloomadel. - Veterinaarmeditsiin 2001, Tartu, 2001, lk.13- 21.

  32. Sudakov M, Häkkinen L. Animal Mycobacterioses in Tallinn Zoo (2000).- International Conference: Current Issues in Veterinary Medicine, Proceedings, 29 September 2000, Jelgava, Latvia, 203-208.

  33.  Sudakov M., Koslov N., Häkkinen L. Kanade tuberkuloos ja loomade mükobakterioosid Eestis (1998).- Veteriaarmeditsiin' 98.- Tartu, 104 - 107.

  34. Sudakov M., Nikopensius T., Häkkinen L. Mycobacterium avium complex infection of animals in Estonia - epidemiology and PCR diagnostics (2000).- Feeding, metabolism and infections in farm animals with special reference on reproduction: Proceeding from symposium at Estonian Agriculturale University, Tartu, Februari 24-25, CRU Report 11, 40-45.

  35. Sudakov, M., Nikopensius, T., Häkkinen, L. (1999). Application of molecular (PCR, DNA probe) and conventional methods in animal mycobacterioses diagnostics.- Veterinaarmeditsiin’ 99, Tartu, pp. 87-92 (in Estonian).

  36. Sudakov M., Nikopensius T., Häkkinen L. Mycobacterium avium complex infection of animals in Estonia - epidemiology and PCR diagnostics.- Feeding, metabolism and infections in farm animals with special reference on reproduction: Proceeding from symposium at Estonian Agriculturale University, Tartu Februari 24-25, Uppsala 2000, CRU Report 11, 40-45.

  37. Sudakov M, Häkkinen L. Animal Mycobacterioses in Tallinn Zoo.- International Conference: Current Issues in Veterinary Medicine, Proceedings, 29 September 2000, Jelgava, Latvia, 203-208.

  38. Sudakov M., Sütt S., Kumar J., Häkkinen L. PCR-identification of mycobacteria isolated from tuberculin-reacting cattle in Estonia. Research for Rural Development: International Scientific Conference Proceedings, 23-25 May 2001, Jelgava, 2001, 139-143.

  39. Sudakov M., Sütt S., Häkkinen L., Kokassaar S. Tuberculosis of birds in Estonia: transmission and diagnostics.- Reproductive Failure in Farm Animals: Proceeding from symposium at Estonian Agricultural University, Tartu, June 14-15, 2001, CRU Report 14, Uppsala, 2001, 65-70.

  40. Sudakov M., Häkkinen L., Sütt S. Mycobacterium avium - mycobactreioses of wild animals. - Veterinaarmeditsiin 2001, Tartu, 2001, lk.13- 21. (in Estonian)

  41. Sudakov M., Sütt S., Kokassaar S., Kumar J., Häkkinen L. Spread of Bovine Mycobacteriosis in Estonia: Results of Bacteriological and PCR Diagnostics.- Veterinaarmeditsiin 2002, Tartu, 2002, 87-92.

  42. Sudakov M. Mycobacterioses.- Zoonoses and Food Hygiene, Tartu 2002, 5-22. (in Estonian)

  43. Sudakov M., Kumar J., Kokassaar S., Häkkinen L. Epidemiology of Avian Tuberculosis in Estonia: 1995 - 2003.- Veterinaarmeditsiin 2003, Tartu, 2003, 81-89.

  44. Sudakov M., Kumar J., Kokassaar S., Häkkinen L. Epidemiology of nontuberculous mycobacteria (NTM) infections of domestic and wild animals in Estonia. - Journal of Agricultural Science, 2003, XIV, 5, 303-311.

  45. Sudakov M. Veterinary immunology, Tartu 2004. (in Estonian)